National Institute of Communicable Diseases
Directorate General of Health Services
Ministry of Health and Family Welfare (GOI)
22, Sham Nath Marg, New Delhi-110 054

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ANNUAL REPORT 1997
 
CONTENTS
1.   Introduction
          Organisation Chart
          NICD Faculty/Administration/Staff
          Budget at a Glance (1997)
2.   Objectives and Functions of the Institute
3.   National Health Programmes
          National Surveillance Programme for Communicable Diseases (NSPCD)
          Guinea Worm Eradication Programme (GWEP)
          Yaws Eradication Programme (YEP)
4.   Scientific and Research Activities
       Headquarters
          Division of AIDS & HIV
          Division fo Biochemistry/Biotechnology
          Division of Epidemiology
          Division of Helminthology
          Division of Microbiology
          Division of Medical Entomology/Vector Control
          Division of Training & Malariology
          Division of Zoonosis
       Branches
          Alwar
          Bangalore
          Calicut
          Coonoor
          Jagdalpur
          Patna
          Rajamundry
          Varanasi
5.   Manpower Development/Training Courses, Workshops, Seminars
6.   Outbreak Investigations
7.   Participation in Conferences, Workshops, Seminars and Symposia
8.   WHO/Other International Fellowships
9.   Membership of Expert Committes/Advisory Panel/Honours
10. Important Visitors during the Year
11. Publications/Presentations
Annexures
          Central Library
          Central Animal Facility

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NICD Branch, Alwar

The National Institute of Communicable Diseases, (NICD) is primarily devoted to research and training in the field of communicable diseases. Advancement and dissemination of knowledge relating to the epidemiology and control of communicable diseases have become an important objectives of the institute. It also guides the state health organisation in their  activities specially in control of communicable diseases.

Field Practice Unit, Alwar was established in the year 1973, with an idea to impart field training to different categories of medical and para medical health personnel admitted to various training courses being conducted at National Institute of Communicable Diseases, Delhi. The Deputy Director is the head of the unit. Main functions of this unit are research, training and service in the field of communicable diseases. This institute has done some modest research work on bacterial, protozoal and helminthic infections in man. This branch of NICD provide the basic diagnostic facilities including Widal test, Malaria Parasite (MP), VDRL, stool & water culture for vibrio cholerae for the people of Alwar and adjoining districts of the Rajasthan State. This Institute also undertakes investigation of outbreaks and formulations of measures to control.

This unit has developed good co-ordination with (NICD) headquarters, state and district health authorities to carry out its activities under the leadership of the Deputy Director (Medical).

Routine Services/Activiites & Maintenance of stock cultures of various animals, pathogens & parasites

Name of test

No. of samples

Positive

Percent

Widal test

Cholera culture

Blood for MP

 

 

 

Coliform count

1361

31

1076

 

 

 

125

570

4

Pv – 324

Pf – 204 &

Mix – 15

 

32

41.88

12.90

50.46

Pv – 59.67

Pf – 37.57

Mix – 2.76

25.6 (Unsatisfactory)

Regular supply of VR fluid media bottles for collecting stool samples & water samples from the most peripheral areas of Alwar, Bharatpur and adjoining districts of the Rajasthan State for cholera culture.

Diagnostic services

Name of test

No. of samples

Positive

Percent

Typhoid & paratyphoid feve widal test

Cholera / Cholera culture

Blood for MP

Coliform count 

1361

 

31

1076

125

570

 

4

543

32

41.88

12.90

50.46

Pv – 59.67

Pf – 37.57

Mix – 2.76

25.6 (Unsatisfactory)

Public health and other bacteriological, biochemical services

In the outbreak situation, this unit regularly provides expert epidemiological assistance for prevention and control of epidemic. Bacteriological services e.g. Widal Test, VDRL Test, Cholera culture, Blood for MP, Coliform count.

Identificaion Services

For the cross confirmation, this unit regularly receives blood slide for MP from Dy. C.M. & H.O. (Malaria) office, Alwar.

Research Projects

1.     An epidemiological study of malaria in rural areas of Alwar district

Aims and objectives of the project are to find out the prevalence and incidence of malaria, its seasonal distribution and to suggest the measures for control and prevention of malaria in the area.

The criteria for the selection of the study area was high API  i.e. 9.45% in the village. This research project has been started in the year 1995.

The observation made during the year 1997 were SPR was 39.64% and SFR 43.34%. All the positive cases were given radical treatment. It is interesting to note that not a single malaria death was reported from the study village.

2.     Vital hepatitis carreer survey in the Alwar district

Viral hepatitis in its different types is prevalent in the Alwar district, and the cases occur throughout the year. Endemicity of the disease is responsible for presence of a large number of carriers. This study is proposed to find out the carrier rate of hepatitis ‘B’ in the district. During the year 1996, a total 427 blood samples were collected from the rural as well as urban areas of the district. Out of the them, 22 blood samples were found positive for HbsAg. The career rate was found 5.15 percent in the district.

Viral hepatitis carrer survey in the Alwar district.

Age group

Urban

Male

Rural

 

Male

 

 

 

 

Female No.

+ve%

No.

+ ve %

Female No.

+ ve%

No.

+ve%

 

 

0-4

11

 

3

 

1

 

0

 

15

 

5-14

41

1 (2.44)

34

3 (8.82)

 

 

 

 

96

6 (6.25)

15-44

94

6 (6.38)

70

2 (2.86)

51

2 (3.92)

26

 

241

10 (4.15)

45 +

36

5 (13.89)

18

1 (5.56)

17

 

4

 

75

6 (8.00)

Total

172

12 (6.59)

125

6 (4.8)

82

4 (4.88)

38

 

427

22 (5.15)

In the year 1997, a total of 155 blood samples were collected for the Viral Hepatitis study. The laboratory results of the above samples were not received. Thus it is not included in the total samples collected for the study.

Distribution of the blood samples collected during the year 1997 for the Viral Hepatitis Carrier

Survey in the Alwar

Age Group

Urban

Rural

Total

 

Male (No./ %)

Female (No. / %)

Female (No. / %)

Male (No./ %)

(No. / %)

0 - 4

2/3.03

4/8.33

2/9.52

-

8/5.16

5 - 14

16/24.24

8/16.66

10/47.61

3/15.00

37/23.87

15 - 44

35/53.03

31/.64.58

9/42.85

14/70.00

89/57.41

45 +

13/19.69

5/10.41

-

-

21/13.54

Total

66

48

21

20

155

3.     An epidemiological surveillance of Acute Flaccid Paralysis (AFP) in the Alwar

The study was carried out to find out wild polio virus circulation in the community in relation to occurrence of a case of acute flaccid paralysis (AFP) and to find out asymptomatic wild polio virus infection in the community which is > 99%.

The existing district health infrastructure i.e. subcentre in the rural areas to district hospital at district level was utilised in receiving information regarding occurrence of poliomyelitis (AFP) case in the district and subsequently in collection of stool samples from the cases. The usefulness of testing stool specimens from acute flaccid paralysis (AFP) contacts has been the subject of considerable recent discussion. The testing of contact stools resulted in an increase case detection by 10%.

Five contact stool samples were also collected from family and neighbourhood contact below the age of 5 years as recommended by WHO.

The stool samples were collected in a autoclaved Plastic Vial specially supplied for the collection of the Poliomyelitis Stool samples from the Himedia Laboratory.

A total 4 suspected cases of poliomyelitis were reported from the district. From each case two stool samples were collected within the time interval of 24 to 48 hours & five family/ neighbourhood contact stool samples were collected for each case.

4.     Effect of mass pulse polio immunisation on polio virus activities

A total 88 stool samples were taken from the children in the age group of 0-5 years. These stool samples were taken before completion of first phase of mass pulse polio imunisation i.e. on 7.12.96. Samples were taken in HBSS media and sent to NICD.

Delhi for polio virus culture 69 stool samples as second stool samples of these children was taken one month after the completion of second phase of mass pulose immunisation i.e. on 9.1.97 and sent to NICD, Delhi for polio virus culture.

Laboratory analysis of first phase stool samples revealed that only one of these samples was found positive for Polio Type II & from the second phase of stool samples three were found positive for Polio Type III, one for Polio Type II & for 38 stool samples results awaited.

5.     Viral hepatitis in Alwar town – a repeat study

Viral hepatitis is a major public health problem in India. Earlier a study was conducted in the year 1994 in which 192 viral hepatitis cases were detected in 70,444 population in the Alwar town. The present study is planned to find out change in the trend of viral hepatitis in Alwar town. This study was started during the month of December, 1996 and was completed by the end of April, 1997. During the month of December, 1996 a population of 17,669 in 6 randomly selected wards has been covered and 125 cases of jaundice have been identified. The incidence of jaundice was 7.07 per one thousand of population. However a lower incidence of jaundice i.e. 2.73 per one thousand population was observed in the study conducted during the year 1994.

During the months of February, 1997 to April, 1997, a population of 56,746 of the Alwar town was covered by house to house survey in the 14 randomly selected wards. A high incidence of Viral Hepatitis was detected in this study i.e. 5.74 per one thousand population.

6.     Field training on epidemiology and control of Malaria

(Enhanced Malaria Control Project – 17th to 21st Nov.’97)

Enhanced Malaria control project of NMEP envisages decentralisation of Malaria Control Plan and its implementation at district level and below. This also includes capacity building, community participation, inter-sectoral approach involving different agencies which are indirectly linked for mosquitogenic problems, NGOs and private practitioners etc. Due emphasis is on the process appraoch with flexibility to modify control strategies according to local conditions since malaria is a local and focal phenomena and thus an epidemiological approach leading to appropriat control strategy is essential.

The modified strategy consists of:

§         Early case detection and prompt treatment (EDPT).

§         Selective vector control.

§         Prediction, early detection and effective response to malaria out breaks.

§         Capacity building at district level.

§         IEC, community participation and intersectoral involvement.

Keeping this in view field exercises was carried out at Alwar (Raj.). Objectives of the Studies were i) Collection of epidemiologial and entomological profile of Alwar district. ii) Knowledge, Attitude, Belief and Practice (KABP) on malaria. iii) Environmental & other factors for malaria.

The participants were divided into seven groups for epidemiological & KABP studies, and into five groups for the purpose of entomological studies. All the samples were examined at NICD, FPU laboratory, Alwar. Briefing and demonstration and a workshop on inter sectoral co-ordination was conducted at FPU, Alwar.

During the field exercise a total of 792 blood slides were taken, laboratory result of the slides are awaited. Knowledge, attitude, belief and practice study in respect of malaria and its control measures was carried out in three villages of Alwar (Barkhera, Baleta & Laxmangarh). Adults above 18 years were interviewed by structured questionnaire. A total of 252 individuals were interviewed.

In vilage Baleta 45 individuals (Males 25 and females 20) were interviewed. Llliteracy was to the tune of 69% (31 out of 45). Amount the 14 literate person 8were primary,4 were middle and 2 in high school. Among the 45 interviewed the occupational break-up was- Agricultural labour –32, Skilled worker –6 and Housewives.Out of 45 interviewees only 4 said that MPW is visiting their house everyu fortnbightly,5 told that the MPW has not visited at all while 36 persons said that the MPW is visiting iregylarly.

Of the persons interviewed, 25 would go to the Dispensary if their familuy mnembers gets fever while 16 would prefer to go to the private practitioners for getting their family members treated for fever and only 4 did not know where to go for getting treated for fever 24interviewees said that they get their house mud painted/plastered every 6 months and monthly respectivel.

In village Barkhera, 83 individuals (Males 49 and females 34) were interviewed. Illitearcy was to the tune of 50% (41 out of 83). Among the 42 literate person 16 were primary, 15 middle and 9 in high school. Among those interviewed the occupational break-up was Agricultural labour- 33,skilled worker –15 and Housewives 32. Out of 83 interviewees 44 said that MPW is visiting their house everyu fortnightly while the others said MWP were either irregular/not visiting at all. Of the persons interviewed 66 would go to the Dispensary if their family members gets fever while 17 would prefer to go to the private practitioners for getting their fgamilyu members treated for fever. In Laxmangarh 124 individuals( Males 63 and females 61) were interviewed. Illiteracy was to the tune of 26% (32 out of 124). Among the 88 literate,person 15 were primary, 30 middle and 45 in high school or above. Among those interviewed, the occupational break-up was- students –5, business –36, labour –15 and housewives 53. Out of 124 interviewees,9 said that MPW is visiting ther house every fortnightly while the others said MPW were either irregular/not visiting at all. Of the persos interviewed 1099 would go to the Dispensary if their famuiluy members gets fever while 7 would prefer to go to the private practitioners for getting their family members treated for fever.

The village named Balana  (Population 4500) under CHC Malakheda of Alwar district Rajasthan was selected for the study. This village is near Jaisamand Lake, which forms a large water reservoir (Lake) for irrigation. The Entomological survey was done from 6 AM to 8 AM on 17.11.97.

The main objectives of the study were:

To demonstrate to the participants anout the vector prevalence, its breeding and resting habits, its breeding behaviour and

To monitor the insecticide susceptinbilityu status against different vonventional insecticides etc. from this identified Malaria Prone and high risk areas of Alwar district.

The people are of low socio-economic status and are mainly agricultural labourers. The village is having a numbher of sepages form the reservior and a variety of breeding grounds providing high mosquitogenetic potential and prone to Malaria. There is no transmigration of the population as the people are inflected with agricultural activities. However being a project area, sufficient irrigation facilities have been provided to the farmers.

Mosquito Prevalence:  During the survey a total of five anopheles species were encountered from this village, namely An. Culicifacies, An annularis, An Splendidis, An Subpictus and An., Aconitus.  A total of 52 houses (Human dwelling)were covered for the adult mosquito collections. The area falls under DDT spray since very long, where the vector An. culicifacies is susceptible to nboth DDT and Malathion. This year, only one round of DDT was sprayed , as there were several finacial and administrativbe constraints for the second round.

Larval survey : In order to detect the different nbreeding places, an intensive searcxh was carried out.A total of at least 58 breeding places were searched out of which 32 breeding were found positive for anopheles and culex breediong which contrinute 55.2% for moisquito breeding. The seepage water was coming out of the dam from samll streams having marginal aquatic vegetations all along the anopheles mosquitoes. However no An. fluviatities larvae could nbe collected for, these slow moving streams.

Biological control of mosquitoes: In Alwar town, larvivours fishes are introduced tin ornamental water tanks as biological method of mosquito contorl. Because of the presence of these fishes (gambussia affinis) no larve could nbe encountered from these ornam,ental water tanks.

Wall Bio-assay: In order to assess the potencyu of an insecticide deposit for adult mosquitoes at various times after application on different surfaces and then to detect the onset of a definite decline in the toxic effect of the deposit, this techniques was observed by demonstration. It was also explained that supplementay mathods of window-trap collections and survival tests sbe read with Bio-assay tests.

Susceptibility status: Demonstration on the method of determining the susceptibility resistance of adult mosquitoes to organochlorine, organophosphate and carbamate insecticides was carried out. The purpose of this test was thoroughly discussed and the need to conduct such tests to detect the presence of resistant mosquitoes as soon as possible was emphasised so that alternative control plans can be made in time for change of insecticide.

Laboratory studies: The field collected biological specimens were brought to the laboratory and processed for the following demonstration.

§         Identification of different species of mosquitoes.

§         Processing of abdominal blood meal for precipitin test.

§         The working of CDC (Communicable Diseases Centre – Atlanta) Light trap for collection of mosquitoes.

Pyrethrum catch (Total catch) collection window-trap collection & Meagan trap collection etc. were also explained.

Statement showing different species of mosquitoes collected from Balana village

Name of the specie

Total number of people engaged collection

Total hours specie

Total number of mosquito collected

DPMH

 

 

 

Male

Female

 

A.culcifacies

23

23 hrs.

4

44

2.1

A.annularis

-do-

-do-

-

18

0.8

A.splendidus

-do-

-do-

-

14

0.6

A.subpictus

-do-

-do-

7

360

16.0

A.aconitus

-do-

-do-

-

1

0.05

C.quenguifaciatus

-do-

-do-

4

56

3.0

C.vishnui (g)

-do-

-do-

-

9

0.4

Consolidated statement showing the breeding places checked for the presence of mosquito larvae collected from Balana village

Type of breeding places

Total number checked

No. found positive

Average density per dip specieswise

Stagnant seepage water

33

21

1. Anophelis: 12 per dip

2. Culex: 18 per dip

Slow moving stream

8

8

1. Anopheles: 6 per dip

2. Culex: 10 per dip

3. Culex: 10 per dip

Stagnant water in tanks

5

3

1. Anopheles: 12 per dip

2. Culex: 18 per dip

Ornamental tanks

7

-

-

7.     Mass Pulse Polio Immunisation Phase I – 7.12.1997

During the first phase of Mass Pulse Polio Immunisation, two polio vaccination centre were opened, one at FPU, NICD, Alwar and another with the coordination of local private practitioner in the area of Naya Bas, Alwar. A total of 340 OPV dosages were given to children in the age group of 0 to 5 years. This community service is provided in coordination with district health authority to achieve the goal of “A WORLD FREE OF POLIO” by 2000 A.D. as targeted by member countries of WHO.

8.     An epidemiological study of measles in Alwar town

Measles is endemic in all parts of the INdia & is a major cause of morbidity and a significant contributor to childhood mortality. This study was conducted in the Alwar town.

§         To find the incidence of measles in the Alwar town.

§         To find out the factors responsible for occurrence of the cases in endemic and epidemic form.

§         To find out the change in measles morbidity and mortality after introduction of measles vaccine in the UIP.

Measles survey in Alwar town – Feb. 1997 to March 1997

S.No.

Ward N

Population

Measles Ca

Blood Samples Tak

Measles Incident 1000 population

1

11

4,824

33

14

6.84

2

14

1,912*

96

53

50.20

3

20

436*

48

24

110.09

4

23

4,433

98

38

22.10

5

25

793*

28

18

35.30

6

27

3,144

141

40

44.84

7

29

3,322

92

30

27.69

8

31

3,201

8

5

2.49

9

35

3,732

80

29

21.43

10

37

4,313

76

30

17.62

11

38

4,082*

83

57

20.33

Total

 

34,192

783

338

22.90

* These wards were partially covered

A total 34,192 population has been covered in randomy selected 11 wards of the Alwar town. Total 783 cases of measles were identified on the basis of history given by mother and that also been supported by typical presentation of the measles case. 338 blood samples were taken over the filter paper strip. The incidence rate of measles was found 22.90 per 1000 population.

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